Fig 1: A genetic screen in Drosophila identified actin cytoskeleton-related YARS1CMT interactors.a A schematic representation of the principles of a genetic screen in which ommatidial disorganization of eyes in adult fruit flies was used as readout. GMR-Gal4 > YARS1CMT expressing flies (orange) were crossed to individual EP lines (brown). Genuine YARS1CMT modifier (purple) would induce disorganized ommatidia only in the mutant background and will not have an effect when crossed to YARS1WT-expressing flies in a validation step (blue). See also a detailed description in the Methods. Scanning electron micrographs of adult fly eyes in b controls, and c upon co-expression of FimEP. Assessment of interaction with transgenic UAS-Fim (d), and the human orthologs UAS-PLS2 (e) and UAS-PLS3 (f) with YARS1WT or YARS1E196K. The experiments were repeated at least three independent times with similar outcomes. Scale bars – 50 µm.